Table 2.
Quantitative image analysis of the histochemical and imnunohistochemical determinations, and other in the kidney of ZDFR treated long-term with a low dose pioglitazone
| Rat strain | LZR | ZDFR | ZDFR |
|---|---|---|---|
| Pioglitazone dose | – | – | Low |
| Treatment (months) | 4 | 4 | 4 |
| Age (months) | 7 | 7 | 7 |
| Body weight (g) | 379 ± 19* | 486 ± 13 | 490 ± 12 |
| Glycaemia (mg/dl) | 105 ± 7* | 455 ± 32** | 243 ± 21 |
| GS (%)/glomerulus | 1.0 ± 0.4* | 27.1 ± 5.1** | 7.6 ± 2.2 |
| IF (%)/cross section | 2.4 ± 1.2* | 15.9 ± 3.0** | 6.0 ± 1.6 |
| TA (%)/cross section | 1.5 ± 0.8* | 8.7 ± 2.2** | 4.2 ± 1.2 |
| αSMA (%)/cross section | 1.2 ± 0.5* | 13.3 ± 1.9** | 4.5 ± 1.2 |
| Nephrin (%)/per glomerulus | 20.4 ± 2.4* | 5.1 ± 1.3** | 16.9 ± 2.3 |
| Haem oxygenase 1 | 10.6 ± 1.8* | 66.2 ± 12.3** | 20.4 ± 3.9 |
| GSH/GSSG ratio | 8.8 ± 0.9* | 3.1 ± 0.6** | 5.9 ± 0.7 |
| Malonyldialdehyde (nmoles/mg protein) | 128 ± 18.3* | 279 ± 18.7** | 195 ± 12.8 |
| ED1 (CD68) | 1.3 ± 0.9* | 15.3 ± 2.2** | 3.4 ± 1.0 |
GS = glomerulosclerosis; IF = interstitial fibrosis; TA = tubular atrophy; ASMA = α-smooth muscle actin; TGFβ1 = transforming growth factor β1.
All values expressed as means ± SD.
*Versus all groups P < 0.01.
**Versus ZDFR, 7 months, PGT low, P < 0.01.