Fig. 6.

Reconstituting p21 expression in p21 −/− podocytes restores the prosurvival effect of rosuvastatin. Growth-restricted p21 −/− mouse podocytes were stably transfected with a human p21 vector or GFP vector as a control. Western blotting for p21^WAF1/CIP1 +/+ showed successful re-expression of p21 in the p21 −/− podocytes (A). Following stable transfection, cells were pre-incubated with rosuvastatin or DMSO and ADR-induced apoptosis was measured by FACS analysis (B). The prosurvival effects of rosuvastatin in podocytes as a function of p21 status are summarized in (C).