Table. Methods used to diagnose human Rickettsia typhi infection, Yucatan, Mexico, 2007*.
| Sample | PCR, % identity |
IFA, titer† |
|||
|---|---|---|---|---|---|
| Citrate synthase (gltA) (382 bp)‡ | 17-kDa gene (434 bp)§ | IgM | IgG | ||
| Rickettsia typhi Wilmington strain | 100 (AE017197.1) | 100 (M28481.1), 99 (AE017197.1) | 256 | 128 | |
| R. felis URRWXCal2 | NR | 89 (CP000053.1) | ND | ||
| R. rickettsii Sheila Smith | 92 (CP000848.1) | 89 (CP000848.1) | Neg | 128 | |
| R. akari Hartford strain | 91 (CP000847.1) | 96 (CP000847.1) | Neg | 64 | |
*IFA, indirect immunofluorescence assay; Ig, immunoglobulin; NR, not represented in the first 100 sequences with a significant alignment using BLAST (9); ND, not determined; Neg, negative. †Titers refer to the reciprocal of the dilution. ‡Primers: RpCS.1258 5′-attgcaaaaa gtaccgtaaaca-3′ and RpCS.877 5′-gccccgccgtgggcaggccccc-3′. §Primers: 17-kDa 5′-gctcttgcaacttctatgtt-3′ and 5′-cattgttcgtcaggttggcg-3′.