Table 3. Sensorimotor Function Assessments.
Tests | Group | Day 0 | Day 1 | Day 3 | Day 7 | Day 14 |
---|---|---|---|---|---|---|
Neurological score median (25th to 75th percentiles) | WT | 0 | 1 (0.25–1) | 1 (1–2) | 1 (1–2) | 1 (0.25–1) |
Ngb-Tg | 0 | 1 (1–2) | 2 (1.5–2) | 1 (0–1) | 1 (0–1) | |
Rotorod test, % of day 0 | WT | 100 | 70.5±10.8 | 78.5±10.6 | 90.5±9.7 | 104.3±3.3 |
Ngb-Tg | 100 | 68.7±12.8 | 78.1±10.9 | 84.5±17.4 | 110.7±2.5 | |
Hanging wire median (25th to 75th percentiles) | WT | 5 (4.75–5) | 2.5 (1–4) | 3.5 (2–4.25) | 4 (4–5) | 4.5 (4–5) |
Ngb-Tg | 5 (4.5–5) | 3 (1.5–3) | 4 (2.5–4) | 4 (3.5–4) | 4 (4–5) | |
Foot fault test median (25th to 75th percentiles) | WT | 0 (0–1) | 3 (0–5.75) | 4 (0–5.25) | 5 (4–5) | 2 (1–2) |
Ngb-Tg | 1 (0–1.5) | 2 (1–5) | 4 (2–6) | 4 (3.5–4.5) | 1 (1–1.5) | |
Body weight, % of day 0 | WT | 100 | 91.7±0.57 | 82.1±1.21 | 81.8±3.04 | 91.3±1.54 |
Ngb-Tg | 100 | 91.5±0.73 | 80.1±1.58 | 81.2±3.45 | 91.9±1.55 |
Sensorimotor deficits and body weight loss were assessed before (day 0) and at days 1, 3, 7, and 14 after transient (1-hour) focal cerebral ischemia. Results are expressed as median values and 25th to 75th percentiles for assessments of neurological score, hanging wire, and foot fault tests; and mean±SEM for assessments of Rotorod test and body weight loss. N=6 for Ngb-Tg and 7 for WT per group; 23- to 27-g male mice. In all assessments, there were no statistical significant differences between WT and Ngb-Tg groups.