Table I.
Antibodies and conditions used for immunohistochemistry
| Antigen | Source | Species | Dilution | Retrieval | Target |
|---|---|---|---|---|---|
| 3β-HSDa | Gift | Rabbit | 1:800 | N | Leydig cells |
| SMAb | Sigma | Mouse | 1:1000 | N | Peritubular myoid cells |
| Cytokeratin | Santa Cruz | Mouse | 1:500 | Y | Sertoli cells |
| AMHc | Santa Cruz | Goat | 1:1000 | N | Sertoli cells |
| OCT4 | Santa Cruz | Goat | 1:40 | Y | Undifferentiated gonocytes |
| C-KIT | Dako | Rabbit | 1:20 | Y | Undifferentiated gonocytes |
| AP-2γ | Santa Cruz | Mouse | 1:40 | Y | Undifferentiated gonocytes |
| VASA | Abcam | Rabbit | 1:200 | Y | Germ cells |
| N-Cadherin | Zymed | Mouse | 1:200 | Y | Cell adhesion molecule |
| SOX9 | Chemicon | Rabbit | 1:100 | Y | Sertoli cells |
| Ki67 | Dako | Mouse | 1:40 | Y | Proliferating cells |
a3b-Hydroxysteroid dehydrogenase;bsmooth muscle actin; canti-Müllerian hormone.