Figure 6.

Western blots on flow cytometry-sorted B cells for SHIP and C/ebpβ show that miR-155 leukemic pre-B cells have lower expression of these 2 proteins compared with the wild-type counterparts. (A) Immunoblot shows a stepwise down-regulation of the SHIP expression in the pre-B and mature B cells in the leukemic sorted cells compared with their preleukemic and wild-type counterparts. (B) Densitometry for SHIP expression in pre-B cells calculated on the previous immunoblot confirms that SHIP is expressed the most in the wild-type, less in the preleukemic, and the least in the leukemic cells. (C) Immunoblot on sorted splenocytes shows a down-regulation of C/ebpβ in the pre-B cells of leukemic mice compared with their preleukemic and wild-type counterparts. (D) Densitometry for the C/ebpβ expression in different pre-B cells (wild-type, preleukemic, and leukemic), calculated on the previous immunoblot, confirms that C/ebpβ has higher expression in the leukemic pre-B cells than in the preleukemic and wild-type ones.