Table 1.

NF-κB inhibition prevents apoptosis in cytokine-matured DCs treated with IFNβ and in LPS-matured DCs

JSH-23, μM	% Viability
	TNF-α + IL-1β + IL-6 + PGE2 + IFNβ	LPS
0	44 ± 1	61 ± 3
0.1	54 ± 2	66 ± 1
1	71 ± 7	90 ± 1
10	95 ± 1	94 ± 1

CD11c⁺ DCs were pretreated with different concentrations of JSH-23 for 1 hour followed by treatment with the cytokine cocktail (TNF-α + IL-1β + IL-6 + PGE2) in the presence of IFNβ (500 IU/mL), or with LPS (100 ng/mL). A second dose of JSH-23 was added 24 hours later, and apoptosis was assessed 48 hours after the initiation of cultures by flow cytometry after annexin V/PI staining. Medium controls (no treatment) and cells treated with the cytokine cocktail in the absence of IFNβ averaged 90% to 95% viability.