Figure 6.

Internalization of single spanning apical proteins with no known sorting information is impaired in ethanol-treated cells. A, WIF-B cells were treated in the absence (a, c) or presence (b, d) of ethanol (EtOH) and labeled for DPP IV (a, b) or HA (c, d). Asterisks are marking selected BCs. Bar = 10 μm B, WIF-B cells were treated as described in A and the relative distributions determined as described in Figure 1B. Values are expressed as the mean ±SEM. Measurements were done on at least three independent experiments. * P < 0.05; ** P < 0.01 C, Cells were treated in the absence (a) or presence (b) of ethanol (EtOH). Live cells were labeled with anti-DPP IV antibodies and the antigen-antibody complexes were chased for 45 or 90 min in the continued absence or presence ethanol. Cells were labeled with secondary antibodies to detect the trafficked (tr) complexes. Bar = 10 μm