FIGURE 3.

Effects of blocking reagents on cadherin-mediated outgrowth. Dentate granule neurons were grown on purified N-cadherin-Fc (black bars, A), cadherin-8-Fc (gray bars, A), or on L cells expressing N-cadherin (black bars), cadherin-8 (gray bars), or no cadherins (white bars) (B, C) and the effects of the blocking reagents were measured. (A) HAV reduces axon extension on N-cadherin, and QAV increases axon extension on cadherin-8 compared with scrambled peptide (SCR) controls (for A: ANOVA, P < 0.0,001; Tukey's post test, *P < 0.05). The effects of QAV are specific to cadherin-8: QAV has no effect on axon extension on N-cadherin (A), untransfected L cells (B), and similar to the effects observed purified cadherin-8 (A), QAV increases axon extension on cadherin-8 expressing L cells compared with SCR-treated or untreated cadherin-8 L-cells (B). The magnitude of the QAV-mediated increase is similar to that observed on N-cad expressing L-cells (black, B). (for B: ANOVA, P 5 0.0,019; Tukey's post test, *P < 0.05, **P < 0.01). Abbreviations: as in figure 2; SCR, scrambled peptide; QAV, QAV peptide; HAV, HAV peptide.