Figure 5.

Morphine enhanced Akt phosphorylation in a PI3K and μ-opioid receptor-dependent manner. Primary microglial cells were treated with 100 nm morphine for 0, 5, 15, 30, 60, and 120 min, subjected to Western blot analysis and probed with anti-pAkt then Akt antibody (a). b, Image of Western blot membrane of microglia pretreated for 15 min with 0 or 100 nm wortmannin, then treated for 15 min with 0 or 100 nm morphine. c, Image of Western blot membrane of microglia pretreated for 15 min with 0 or 50 μm LY294002, then treated for 15 min with 0 or 100 nm morphine. d, Image of Western blot membrane of microglia pretreated for 15 min with 0 or 100 nm of the μ-opioid receptor antagonist CTAP, then treated for 15 min with 0 or 100 nm morphine.