Figure 6.

Morphine enhances Iba1 expression in microglial cells. Primary microglia were treated with 0, 1, 10, or 100 nm morphine for 6 (a), 12 (b), 24 (c), or 48 (d, e) h, lysed, and subjected to Western blot analysis. a–d, Graphs indicate relative Iba1 expression normalized to β-actin loading control ± SEM. e, Image of membrane after 48 h treatment probed with anti-Iba1 and anti-β-actin antibodies. Graphs show data from at least four individual Western blots, n = 2 per blot. *p < 0.05, **p < 0.01, ***p < 0.001.