Figure 8.

Morphine-induced Iba1 and P2X₄ receptor expression is μ-opioid receptor-dependent. Primary microglia were treated with 0 or 100 nm of the μ-opioid receptor-selective antagonist, CTAP, for 1 h, then treated with 0 or 100 nm morphine for 24 h. Cells were lysed and subjected to Western blot analysis. a, Image of Western blot membrane probed with anti-Iba1 and anti-β-actin antibodies. b, Graphical analysis of CTAP inhibition of morphine-induced Iba expression normalized to β-actin loading control ± SEM. c, Image of Western blot membrane probed with anti-P2X₄ receptor and anti-β-actin antibodies. d, Graphical analysis of CTAP inhibition of morphine-induced P2X₄ receptor expression normalized to β-actin loading control ± SEM. Graphs show data from at least six individual Western blots, n = 1 per blot. *p < 0.05, **p < 0.01, ***p < 0.001.