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Six, twelve, twenty-four, and forty-eight hour morphine treatment dose-dependently enhanced microglial migration. Primary microglial were treated for 2, 6, 12, 24, or 48 h with 0, 1, 10, or 100 nm morphine and then allowed to migrate toward 10 μm ADP. Quantification of microglial migration after 2, 6, 12, 24, or 48 h morphine treatment, represented as mean ± SEM.
