Table 2.
Accumulation of genomic material in nuclei of cap-gs105 mutants, cap-h, and cap-d2 morphants.
| DNA content fraction | |||||
| <2C | 2C | 2C – 4C | 4C | >4C | |
| WT | 2.2% ± 0.3% | 87.9% ± 0.1% | 4.5% ± 0.5% | 3.5% ± 0.4% | 1.3% ± 0.1% |
| cap-gs105 | 6.8% ± 1.7% | 65.8% ± 4.0% | 6.1% ± 0.5% | 15.5% ± 1.3% | 4.2% ± 0.7% |
| MOcap-h | 4.3% ± 1.9% | 70.1% ± 3.7% | 8.2% ± 0.8% | 13.1% ± 0.3% | 2.6% ± 1.0% |
| MOcap-h+p53 | 3.7% ± 1.2% | 74.2% ± 8.7% | 6.6% ± 2.1% | 11.2% ± 3.8% | 3.0% ± 1.2% |
| MOcap-d2 | 9.8% ± 5.4% | 61.4% ± 10.7% | 11.3% ± 3.0% | 10.0% ± 1.5% | 4.7% ± 1.5% |
| MOcap-d2+p53 | 7.6% ± 3.5% | 64.4% ± 14.2% | 9.3% ± 3.2% | 12.2% ± 4.2% | 4.8% ± 2.9% |
| WT vs. cap-gs105 | |||||
| p < 0.05 | p < 0.01 | p < 0.01 | p < 0.001 | p < 0.01 | |
| WT vs. MOcap-h | |||||
| p > 0.05 | p < 0.01 | p < 0.01 | p < 0.001 | p > 0.05 | |
| WT vs. MOcap-d2 | |||||
|
t-test p-values |
|||||
| p > 0.05 | p < 0.05 | p < 0.05 | p < 0.005 | p < 0.05 | |
| MOcap-h vs. MOcap-h+p53 | |||||
| p > 0.05 | p > 0.05 | p > 0.05 | p > 0.05 | p > 0.05 | |
| MOcap-d2 vs. MOcap-d2+p53 | |||||
| p > 0.05 | p > 0.05 | p > 0.05 | p > 0.05 | p > 0.05 | |
Table showing the distribution of nuclear DNA contents among all counted cells. Values represent an average of four independent experiments. t-test p-values (lower table) reveal a significant shift from diploid to aneuploid and especially tetraploid nuclei in cap-gs105, cap-h morphants or cap-d2 morphants compared to wild-type. There are no significant differences in the genomic content distributions of cap-h morphant and cap-h/p53 double morphant or of cap-d2 morphant and cap-d2/p53 double morphant retinae.