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Real-time RT-PCR showed that miR-200 family (A), let-7 family (A), and E-cadherin (B) were significantly down-regulated and vimentin (B) was up-regulated in gemcitabine-resistant PC cells. Western Blot analysis (C) showed that ZEB1 and vimentin protein expression was up-regulated and E-cadherin was down-regulated in gemcitabine-resistant PC cells.
