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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Exp Hematol. 2009 Jun 6;37(9):1038–1053. doi: 10.1016/j.exphem.2009.05.011

Figure 4. VEGF mRNA levels and protein secretion are reduced in G4N and G6N EBs.

Figure 4

Total RNA was isolated from day 6 EBs. RNA was reverse transcribed and real-time PCR analysis was performed using the ABI 7500 for Vegf relative quantification. Error bars represent s.e.m. *p<0.05 (A). Supernatant from day 8 EBs was collected, and the concentration of VEGF protein was determined by ELISA (representative experiment) (B).