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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Exp Hematol. 2009 Jun 6;37(9):1038–1053. doi: 10.1016/j.exphem.2009.05.011

Figure 5. Schematic diagram of VEGF and Flk-1 Fc treatment of EB cultures.

Figure 5

In rescue experiments, EBs were treated with VEGF (2.5, 5 and 10 ng/mL) on EB differentiation day 6 and allowed to differentiate for 2 additional days. In blocking experiments, 5 ng/mL of VEGF and soluble Flk-1 Fc (5, 10, 20 ng/mL) were added to the culture medium on EB differentiation day 6 and EBs were differentiated for 2 additional days. In both cases, EBs were dissociated into single cells on day 8 and plated into definitive hematopoietic progenitor assays.