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. Author manuscript; available in PMC: 2009 Dec 11.
Published in final edited form as: Nature. 2009 Jun 11;459(7248):861–865. doi: 10.1038/nature08063

Figure 2. Phenotypic analysis of nas6Δ, hsm3Δ, and rpn14Δ mutants.

Figure 2

a, Strains with indicated genes deleted were spotted on plates in four-fold dilutions and grown at the indicated temperature.

b, Cell lysates (75µg) were resolved on native gels. Gels were stained for LLVY-AMC hydrolytic activity.

c, Cell lysates of wild-type or nas6Δ rpn14Δ strains were resolved by two-dimensional native-SDS/PAGE gel electrophoresis followed by immunoblotting.

d, Cell lysates were resolved on 5.25% native gels and immunoblotted. BP1 assignment based on ref. 2. *, background band.