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. Author manuscript; available in PMC: 2009 Aug 16.
Published in final edited form as: J Cell Sci. 2008 May 13;121(Pt 11):1832–1840. doi: 10.1242/jcs.020321

Fig. 4.

Fig. 4

Membrane requirements for R4op and Ii insertion into PKRM.

R4op and Ii were synthesized in the RRL. Puromycin/high salt washed membranes (PKRM), mock treated (PKRM mock) or membranes treated with 1 or 2 μg/ml trypsin (PKRM-T 1 and PKRM-T 2) were present during the synthesis of Ii. R4op was incubated with these membranes post-translationally. Where indicated, 100nm of soluble SRP receptor (SR) was added. Proteins were analyzed by SDS-PAGE and autoradiography. Ii*: nonglycosylated Ii; g-R4op: glycosylated R4op.