Table 1. Summary of immunological and physiological genes of interest with significant differences in expression between control and infected frogs.
Function | Tissue | Time-Point | Average Fold Change | JGI Gene ID | Ensembl Gene ID |
Coagulation factor | liver | early | −4 | 279909, 293803, 330213, 341521, 341530, 341561 | ENSXETG00000002964, ENSXETG00000002769 |
Coagulation factor | liver | late | −5 | 147254, 279909, 292258, 292259, 293803, 330213, 341521, 341530, 341561 | ENSXETG00000002964, ENSXETG00000002769, ENSXETG00000003472, ENSXETG00000025492, ENSXETG00000025493 |
Complement component (alternative pathway) | liver | early | −6 | 308546, 327877, 420558 | ENSXETG00000005261, ENSXETG00000010935, ENSXETG00000024110 |
Complement component (membrane attack complex) | liver | early | −6 | 282192, 295244 | ENSXETG00000003532, ENSXETG00000006766 |
Complement component (classical pathway) | liver | early | −3 | 399987, 471107 | ENSXETG00000005203, ENSXETG00000027636 |
Complement component (classical pathway) | liver | late | −2 | 399976, 399987,400010, 420551 | ENSXETG00000010930, ENSXETG00000023914, ENSXETG00000027636 |
Cytochrome Cyp450 | liver | early | −3 | 113474, 297805, 325065, 448177, 464259 | ENSXETG00000005868, ENSXETG00000006924, ENSXETG00000013846, ENSXETG00000016349, ENSXETG00000024804, ENSXETG00000027605 |
Cytochrome Cyp450 | liver | late | −4 | >50 genes, see Table S1 | >50 genes, see Table S1 |
Epoxide hydrolase | liver | late | −4 | 126998, 152658, 398826 | ENSXETG00000017581, ENSXETG00000022654, ENSXETG00000028064 |
Heat shock protein 70 | liver | late | 4 | 268191, 287936, 361977, 456096 | ENSXETG00000006714, ENSXETG00000008828, ENSXETG00000010075, ENSXETG00000016838 |
Heat shock protein 70 | skin | late | 6 | 361977, 456096 | ENSXETG00000006714, ENSXETG00000008828 |
Keratin, type I | skin | late | 10 | 178963 | ENSXETG00000012482 |
NF-kappa inhibitor | liver | late | 3 | 309572, 396637, 309732 | ENSXETG00000006998, ENSXETG00000012114 |
Ornithine decarboxylase | skin | late | 18 | 196068, 284956, 295202 | ENSXETG00000003519, ENSXETG00000007373 |
Small chemokine, interleukin 8 | liver | late | −3 | 295905 | NA |
Because multiple genes with similar function were often perturbed, fold change is given as an average for all differentially expressed genes in each category. Genes with increased and decreased expression in Bd-exposed frogs show positive and negative fold changes, respectively. See text for additional genes of interest and Table S1 for a complete gene list of differentially expressed genes.