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. Author manuscript; available in PMC: 2010 Aug 15.
Published in final edited form as: Cancer Res. 2009 Aug 15;69(16):6668–6675. doi: 10.1158/0008-5472.CAN-09-1284

Figure 1. CUL1-positive maternal centrioles serve as assembly platforms for oncogene-induced centriole overduplication.

Figure 1

(A) Immunofluorescence microscopic analysis for CUL1 using U-2 OS cells stably expressing centrin-GFP (U-2 OS/centrin-GFP). Arrowheads indicate centrioles shown in inserts. Nuclei stained with DAPI. Scale bar indicates 10 μm.

(B) Co-immunofluorescence microscopic analysis of U-2 OS/centrin-GFP cells for CUL1 and CEP170, a marker for mature maternal centrioles. Arrowheads indicate centrioles with co-localization of CUL1 and CEP170 (see also inserts).

(C) Immunofluorescence microscopic analysis of U-2 OS/centrin-GFP cells for CUL1 following overexpression of E2F-1. Note overduplication of centrioles in the presence of only two CUL1-positive centrioles (bottom panels).

(D) Quantification of the proportion of U-2 OS/centrin-GFP cells with centriole overduplication in the presence of one or two CUL1-positive centrioles after overexpression of c-MYC, HPV-16 E7 or E2F-1. Arrows point to centrioles shown in inserts. Mean and standard error of three independent experiments with at least 100 cells counted per experiment are shown.