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. Author manuscript; available in PMC: 2010 Aug 15.
Published in final edited form as: Cancer Res. 2009 Aug 15;69(16):6668–6675. doi: 10.1158/0008-5472.CAN-09-1284

Figure 4. PLK4 is degraded by the proteasome.

Figure 4

(A) Immunoblot analysis of whole cell extracts from U-2 OS/centrin-GFP cells transiently transfected with either empty vector (control) or PLK4 and treated with 1 μM of the proteasome inhibitor Z-L3VS or 0.1% DMSO at 24 h after transfection for an additional 24 h.

(B) Immunofluorescence microscopic analysis or U-2 OS/centrin-GFP cells treated with 0.1% DMSO or 1 μM Z-L3VS for 48 h and stained for PLK4. Note centriole multiplication together with an excessive amount of PLK4 at maternal centrioles in Z-L3VS-treated cells