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. Author manuscript; available in PMC: 2010 Feb 15.
Published in final edited form as: Cancer Res. 2009 Aug 15;69(16):6405–6413. doi: 10.1158/0008-5472.CAN-09-1063

Figure 2. Induction of senescence-like cell cycle arrest by long-term downregulation of DEK.

Figure 2

A, Total cell numbers estimated in SK-MEL-28 (left panel) and SK-MEL-94 (right panel) after infection with control shRNA or shDEK-2 (shDEK)-expressing lentiviruses. Error bars represent SEM. B, melanoma cell lines were transduced as in A, and fifteen days after infection, cell cycle profile distribution was analyzed by flow cytometry (upper panels), and senescence features were determined by SA-β-Gal activity (blue signal in bottom panels). C, Cells were treated as in A, and whole cell lysates were processed for detection of the indicated proteins by immunoblotting. NT, no treatment

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