Figure 1. Western analysis of 2D PAGE of S. pombe proteins.

A. 50 µg of a wild-type total cell extract was separated by IEF (pH 3–6) followed by SDS-PAGE (12.5%) and Western blotted with anti-SUMO antisera. Boxed region is an expanded version of a longer exposure of the same blot. B. Comparison of species in extracts from wt, pmt3-GG and wt extracts+CIP (5 units/50 µg protein). * presumed forms of SUMO monomer, A,B, forms not observed after CIP treatment , # possible acetylated form.