Figure 3. Effect of pmt3 mutations on levels of sumoylated species and free SUMO/Pmt3 in vivo.

A. Western blot 7.5% SDS PAG of total cell extracts from wt, pli1-d and pmt3 mutant cells as indicated, probed with anti-SUMO antisera (upper panel) and anti-tubulin antisera (lower panel). B. Western blot 12.5% SDS PAG of total cell extracts from wt, pli1-d and pmt3 mutant cells as indicated, probed with anti-SUMO antisera (upper panel) and anti-tubulin antisera (lower panel).