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A. Western analysis of total cell extracts from cells containing mutant versions of SUMO/Pmt3. Lane 1,5 wt, lane 2 pmt3-K14R, lane 3 pmt3-K30R, lane 4 pmt3-K14R,K30R, lane 6 pli1-d, lane 7 hus5-62. B. Morphology of methanol fixed cells, stained with DAPI and calcofluor. C. Phenotype of pmt3 mutants. 10 µl of 10 fold serially diluted cultures were plated onto media as indicated, and incubated at 25°C for 5 days.
