Figure 6.
Production of IFN-γ and IL-17 in the inflamed CNS in the absence of B7-H1 or B7-DC. (a) Wildtype (WT), B7-DC or B7-H1 KO mice were immunized with PLP178–191/CFA and monitored for clinical disease. The data are expressed as the mean clinical score versus days post-immunization (n=10/group). *Mean disease score significantly higher than WT control mice, p<0.05. Results are representative of three separate experiments. (b) CNS mononuclear cells were recovered on days 20–21, pooled (WT: n=3; KO: n=7) and stained for CD90.2 and intracellular IL-17 and IFN-γ Mean numbers of IL-17+ or IFN-γ+CD90.2+ T cells per mouse ± SEM compiled from 2 separate experiments are shown. *Mean number of IFN-γ+ cells significantly higher than in WT controls, p< 0.01.