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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Insect Biochem Mol Biol. 2008 Oct 17;39(1):31–37. doi: 10.1016/j.ibmb.2008.09.010

Fig. 2. Enantioselectivity of rAaJHAMT.

Fig. 2

Chiral-HPLC analysis was performed with (A) racemic JH III, and (B) metabolites from racemic JHA III incubated with the purified rAaJHAMT protein. Arrows indicate (10S)-JH III (S: retention time 13.4 min) and (10R)-JH III (R: retention time 15.5 min), respectively. The S:R ratio of the racemic JHA III used as the substrate should have been ∼50:50 (data not shown), as the JHA III was synthesized by alkaline hydrolysis from the racemic JH III showing 50:50 S:R ratio in (A). The JH III (0.83 µg) produced from racemic JHA III (5 µg) contained almost only (10R)-JH III (>99.5%) and trace amount of (10S)-JH III (B).