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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Dev Cell. 2008 Sep;15(3):478–485. doi: 10.1016/j.devcel.2008.07.007

Figure 4. Cdk1 phosphorylation of Vac17 regulates vacuole inheritance via increasing the interaction of Vac17 and Myo2.

Figure 4

A. Mutation of the four Cdk1 consensus sites of Vac17 results in a partial defect in vacuole inheritance. Vacuole inheritance of wild type and vac17-4A cells were scored at each time point as described in Figure 1D. Two independent cell cycles shown. B. Mutation of four Cdk1 consensus sites in Vac17 does not affect the cell cycle. Cell cycle progression was estimated from the bud-mother index (BMI, ratio of bud to mother cell diameter). >80 cells scored for each time point. Two independent experiments shown. C. Vacuole inheritance is further impaired in the myo2-3A vac17-4A double mutant. >200 cells scored for each sample. Averages and standard deviations from three independent experiments. D. Substitution of the Cdk1 sites of Vac17 decrease its binding to Myo2. Myo2-GFP was immunoprecipitated with an anti-GFP antibody. Co-precipitation of Vac17-HA or vac17-4A-HA assayed by immunoblot. C. Substitution of the Cdk1 sites of Vac17 does not affect its ability to bind Vac8. Vac17-GFP or vac17-4A-GFP was immunoprecipitated with an anti-GFP antibody. Co-precipitation of Vac8 assayed by immunoblot. Asterisks: non-specific bands.