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. 2008 Jun 11;28(24):6079–6091. doi: 10.1523/JNEUROSCI.1170-08.2008

Figure 5.

Figure 5.

Kalirin-7 colocalizes and interacts with N-cadherin adhesion complexes. a, Kalirin-7 partially colocalizes with α-N-catenin. b, Colocalization of N-catenin and kalirin-7 in spine-like structures (arrows). c, Kalirin-7 partially colocalizes with N-cadherin in dendrites (arrows). d, Exogenous kalirin-7, AF-6, and N-cadherin colocalize at the plasma membrane of HEK293 cells. e, Activation or inhibition of N-cadherin adhesion modulates the interactions among AF-6, kalirin-7, and N-cadherin in neurons. Coimmunoprecipitation was done with N-cadherin, AF-6, or kalirin-7 antibodies, respectively; all immunoblots were probed for N-cadherin, AF-6, and kalirin-7. N-cadherin activation causes increased interaction among N-cadherin, AF-6, and kalirin-7; inhibition of N-cadherin reduces interaction among N-cadherin, AF-6, and kalirin-7. f, N-cadherin and kalirin-7 coimmunoprecipitate from rat forebrain homogenates. Scale bars: a, 25 μm; b, 10 μm; c, d, 5 μm.