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dPAGE analysis of the reaction of Duplex F with ECH optical isomers. Lane 1, DEB; lane 2, control (unmodified) DNA; lane 3, R-ECH; lane 4, racemic ECH; lane 5, S-ECH. Note that to compensate for the greater efficiency of DEB cross-linking, half as many counts of the DEB reaction was loaded compared to that for the ECH reactions. ECH reactions were 4 h and pH 5.0.
