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. Author manuscript; available in PMC: 2009 Aug 17.
Published in final edited form as: Anal Chem. 2009 Jun 1;81(11):4510–4516. doi: 10.1021/ac900512x

Figure 5.

Figure 5

Natural convection-driven micromixing and PCR in a single microfluidic chamber. (A) Pumpless sample transport by capillarity. Solution A is a DNA template and solution B is a reaction mixture of primer, enzyme, and dNTPs. (B) Time sequence of heating used for the AH mode micromixing and the PCR process. The inset shows a single heat cycle of the PCR process with a time interval of 1 sec between the adjacent data points. PCR-based amplification of a DNA fragment from the influenza viral strain A/LA/1/87 is performed for 10, 20, and 30 cycles. (C) Influence of PCR cycles. Lanes 1, 2, and 3 correspond to the amplified PCR products after the microfluidic mixing and the subsequent 10, 20, and 30 PCR cycles, respectively. (D) Improvement of PCR by microfluidic mixing. Both lane 1 and lane 2 are the amplified PCR products after the 20 cycle. However, lane 1 is a control without a microfluidic mixing process at AH mode.