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. Author manuscript; available in PMC: 2009 Aug 17.
Published in final edited form as: Virology. 2007 Oct 24;371(1):86–97. doi: 10.1016/j.virol.2007.09.015

Fig. 3.

Fig. 3

The Vpu protein is expressed in C8166 cells infected with SHIVSCVpu. C8166 cells were infected with SHIVSCVpu or SHIVKU-1bMC33 for 5 days. The cells were starved for methionine/cysteine for 2 h and then radiolabeled for 12 h with 35S-methionine/cysteine. Cell lysates were prepared as described in the text and Vpu proteins immunoprecipitated with an antisera generated against the cytoplasmic domain of the subtype B Vpu protein or the subtype C Vpu protein. (Lane 1) Vpu proteins immunoprecipitated from SHIVKU-1bMC33-inoculated cultures. (Lane 2) Vpu proteins immunoprecipitated from SHIVSCVpu-inoculated cultures. (Lane 3) Vpu proteins immunoprecipitated from uninfected cultures.