The genotypes used in this study, their locations of origin, by place name, latitude (N) and longitude (negative sign indicates W, positive E), and elevation in metres. ‘CS nos’ are standard ABRC stock numbers and ‘Accession’ names are from the stock list. Col-0 through Cvi-0 were used to assess natural genetic variation in temperature gradient Hsp101 expression response curves (experiment 1). Col-0, Ler-2 and the hot1-3 null mutants were used to assess fitness effects of Hsp101 functionality and, with Mt-0 and Sha, to assess effects of tissue Hsp101 content on fitness and phenotypic traits