H1	Populations of diverse geographical origin vary in Hsp101 expression. We grew 10 genotypes from the ‘ecotypes’ collection at www.arabidopsis.org and quantified Hsp101 expression in each ecotype across a gradient of Hsp101 induction temperatures. We further characterized the geographical pattern by regressing Hsp101 expression on latitude of origin.
H2	Hsp101 expression affects fitness. We first test for the effect of complete lack of Hsp101 expression on lifetime fecundity by comparing standard laboratory genotypes to their Hsp101 null mutants. We then test how the differences in quantity of Hsp101 expressed by six genotypes influences fecundity.
H3	The fitness effects of Hsp101 depend on genetic background. We addressed this hypothesis by assessing the effects of the Hsp101 null mutation on fecundity in two different genetic backgrounds.
H4	Hsp101 expression has pleiotropic effects on multiple plant traits. We examined the effect of varying Hsp101 expression on a suite of 12 plant traits, including fecundity. For this hypothesis, we measured all traits on the standard laboratory genotypes used in hypotheses 2 and 3.