. Author manuscript; available in PMC: 2010 Jun 1.

Published in final edited form as: Matrix Biol. 2009 Apr 16;28(5):292–303. doi: 10.1016/j.matbio.2009.04.004

Table 1.

List of PCR primer pairs

			Product size (bp)
Target	Primer Pairs^a	Primer sequence (5′ → 3′)	AMBN^WT	AMBN^Δ5–6
Primer pairs used for AMBN mRNA characterization
Exons 2–5	F5	CTGGGAGCACAGTGAATGTC	211	211
	R5	GAGAAAGTGCGTTGAGTCC
Exons 2–8	F5	CTGGGAGCACAGTGAATGTC	571 and 616	265
	R4	CGATCTGGAACACGGTTG
Exons 3–5	F6	CTGTTCCTGTCCCTAGTG	151	151
	R5	GAGAAAGTGCGTTGAGTCC
Exons 3–8	F6	CTGTTCCTGTCCCTAGTG	511 and 556	205
	R4	CGATCTGGAACACGGTTG
Exons 9–11	F2	GGACCAATGGCACACAAC	651	651
	R2	TCAGGGCTCTTGGAAACGC
Primer pairs used for mice genotyping
Exon 5	F7	ACCCATTCCACACAAACCTG	187
	R6	TGTTCCCTTGGTCCTATCC
Neomycin	neo-F1	GGTGGAGAGGCTATTCGGC		540
	neo-R2	TTCGGCAAGCAGGCATCGC

F stands for forward (5′-side region of DNA) and R for reverse (3′-side).