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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Mol Immunol. 2009 Jun 21;46(13):2655–2665. doi: 10.1016/j.molimm.2009.04.015

Figure 1. Peritoneal and splenic B-1 cells are hyporesponsive to BCR mediated proliferation.

Figure 1

(A) T cell-depleted splenic and FACS purified peritoneal B-1a and B-1b B cells from C57BL/6 mice were cultured at a cell density of 2.0 × 105 cells/well with anti-IgM F (ab′)2 or anti-CD40 for 48 hours with indicated mitogens. Shown are the mean proliferation + SE from triplicate set of wells.

(B) FACS purified splenic B-1 (B220+ CD43+) and B-2 (B220+ CD43−) cells were plated at 2.5 × 105 cells/well, treated with the stimulants at the indicated concentrations, cultured for 48 hours and measured for proliferative response by 3[H] Thymidine incorporation. The graph represents one of three independent assays.

(C) T cell-depleted VH12 transgenic splenic B-1 cells and negative control littermates were plated at a cell density of 2.5 × 105 cells/well and cultured for 48 hours as in (B). Graph represents one of three independent assays. * indicates p < 0.01 when comparing B-1 and B-2 cell responses.