Skip to main content
. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Mol Immunol. 2009 Jun 21;46(13):2655–2665. doi: 10.1016/j.molimm.2009.04.015

Figure 4. The Ca2+flux generated by co-cross-linking BCR and CD19 on wild type splenic B cells is PI3K dependent in both B-1 and B-2 subsets.

Figure 4

Splenic B cells were treated with 0.5 μg/ml biotinylated anti-IgM and 2 μg/ml biotinylated anti-CD19 and 10 μg/ml avidin was added to crosslink IgM and CD19. 25 nM wortmannin was added at room temperature 25 min prior to analysis, as described in the Methods section. p < 0.05 comparing the response from B-2 cells without and with wortmannin. This is a representation of at least 3 independent experiments.