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. Author manuscript; available in PMC: 2009 Aug 17.
Published in final edited form as: Mol Psychiatry. 2008 Sep 2;13(12):1138–1069. doi: 10.1038/mp.2008.101

Figure 5.

Figure 5

Figure 5

DISC1 binds to ATF4/CREB2 and N-CoR, and modulates CRE-mediated gene transcription. (A) Exogenous expression of DISC1 suppresses Gal4-ATF4/CREB2-mediated transcription. (B) Interaction of DISC1 and ATF4/CREB2 demonstrated by in vitro binding experiments with purified recombinant His-ATF4/CREB2 and GST-DISC1 proteins. (C) Requirement of the LZ domain of DISC1 for protein interaction of DISC1 and ATF4/CREB2 as demonstrated by co-immunoprecipitation of ATF4/CREB2 and several types of DISC1 deletion mutants with an HA-tag in HeLa cells. An anti-ATF4/CREB2 antibody was used for immunoprecipitation, and an anti-HA antibody for detection. (D) Importance of exon 12 of DISC1 for interaction of DISC1 and N-CoR is indicated by co-immunoprecipitation. An anti-N-CoR antibody is used for precipitation, and an anti-HA antibody for detection of immunoprecipitates by Western blotting. (E) Exogenous expression of DISC1, but not mutant DISC1 with deletion of LZ, augments ATF4/CREB2-mediated repression of CRE-dependent gene transcription.