(A) RNAi constructs and GFP expression vectors were electroporated into the ventricular zone (VZ) at E15 and analyzed at P0. In brains with control RNAi (Con RNAi), 40% of GFP-labeled cells exited the VZ, and 25% of GFP-labeled cells completed migration and formed the superficial layers of the cortex that correspond to bins 9 and 10. By contrast, only less than 15% of GFP-positive cells reached the superficial layers in brain slices with DISC1 RNAi, BBS4 RNAi, or PCM1 RNAi, with the majority of GFP-positive cells remaining in the intermediate zone (IZ), subventricular zone (SVZ), and VZ. Green, cells co-transfected with GFP and RNAi constructs; purple, propidium iodide (PI). Scale bar: 100 µm.
(B) A migration distance is shown. Silencing of DISC1, BBS4, or PCM1 induces delayed radial migration (** P<0.0001). Silencing of both DISC1 and BBS4 expression leads to a more severe defect compared with that with either DISC1 RNAi or BBS4 RNAi. * P<0.05. Values are mean ± SEM.