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. Author manuscript; available in PMC: 2009 Aug 17.
Published in final edited form as: Eur J Lipid Sci Technol. 2009;111(1):39–52. doi: 10.1002/ejlt.200800117

Figure 7.

Figure 7

Two-dimensional mass spectrometric analyses of ceramide molecular species from a lipid extract of human brain temporal cerebellar white matter in a shotgun sphingolipidomics approach. The lipid sample from human temporal white matter for shotgun sphingolipidomics was prepared as illustrated in Fig. 3 in the presence of 1 nmol C17:1 ceramide/mg protein. A conventional ESI mass spectrum in the negative-ion mode was acquired prior to analysis of the building blocks of ceramide molecular species by NL scanning. These building blocks of ceramide molecular species include sphingoid bases of sphingosine (NL 256.2 and NL 327.3), sphinganine (NL 258.2 and NL 329.3), and C20-sphingoid base (NL 284.3 and NL 355.3) with or without the presence of a hydroxyl group in the fatty amide chains as previously described [74]. IS denotes internal standard. All mass spectra are displayed after normalization to the base peak in each individual spectrum.