Table 3. Mutations that suppress the temperature sensitive phenotype caused by dnaBK85E (dnaB134ts).

	Suppressor mutation1	Representative strain (#isolates sequenced; total #in group)2
1	dnaBS371P	MER505 (5; 6)
2	dnaBH65Y	MER512 (1; 5)
3	dnaBS151P	MER524 (1; 6)
4	dnaBA164V	MER510 (2; 6)
5	dnaBE288K	MER517 (3; 6)
6	likely in rrnO	MER4983

¹All strains are derived from KPL69 and contain the indicated suppressor, dnaB134ts (dnaBK85E) and the linked zhb83::Tn917 (mls).

²One representative strain is indicated, along with the total number of independent isolates that were sequenced. In addition, the second number in parentheses indicates the total number of mutants with a phenotype indistinguishable from that of the sequenced representatives.

³The suppressor mutation in this strain appears to be in the rrnO operon (which includes trnO-ala and trnO-ile). There were four suppressors in this class. The suppressor mutation in the strain indicated (MER498) was linked to the rrnO operon and a clone of the operon suppressed dnaB134ts when integrated into the genome. The suppressors in the other three strains were neither cloned nor tested for linkage. The sequence of each of the two tRNA genes in all four suppressors was wild type, indicating that the mutation, at least the one cloned, is most likely in an rRNA gene in the rrnO operon.