Figure 6. IκBζ mainly localizes to the nucleus.

KG-1 cells (10⁶/ml) were stimulated with rIL-1β, rIL-18, rTNFα (10 ng/ml each), or different combinations of these cytokines for 8 h. Cells were harvested for subsequent cytosol and nuclear extraction. Protein-matched nuclear and cytosolic extracts were then analyzed by Western blotting with anti-serum against IκBζ, laminB1 Ab (nuclear marker) and IL-1β Ab (cytosolic marker). Results are representative of 3 separate experiments.