Figure 2. GR binds to the MMTV promoter equally in the presence of Dex±iAs.

(A) ChIP analysis of GR interaction with NucB. Cells were treated with 5 nMDex±8 µM iAs and ChIP assays were done with antibody to GR. NucB bound by GR was quantified by qPCR or by conventional PCR. n = 7–8 independent experiments. (B) EMSAs done with NEs from 1470.2 cells that were untreated (lane 2–0) or treated with 50 nM Dex±8 µM iAs for 30 minutes. [³²P]-labeled consensus GRE±5, 15, and 30-fold molar excess unlabeled competitor GRE visualized as shifted band by PhosphorImager analysis. The Inset graph shows the competition quantified (shifted probe/(Free+shifted)) from each reaction shown.