Table.

Test performance calculated according to different reading techniques (at 450 nm or double reading at 450/655 nm or by direct visual reading without use of plate reader) and to different cut off values as suggested by manufacturer

Reading technique	Cut off according to manufacturer	Result				Sensitivity (%) (95% CI)	Specificity (%) (95% CI)	Accuracy (%) (95% CI)	Likelihood ratio (95% CI)		No of cases in grey zone*
		True positive	False negative	True negative	False positive
Positive	Negative
Reading at 450 nm	Negative <0.140, grey zone 0.140-0.160, positive >0.160	52	0	136	10	100 (93.2 to 100)	93 (87.8 to 96.7)	95 (90.9 to 97.6)	15 (7.6 to 30.3)	0 (0 to 0.08)	H pylori positive=0†H pylori negative=4
Reading at 450/655 nm	Negative <0.100, grey zone 0.100-0.120, positive >0.120	52	0	136	10	100 (93.2 to 100)	93 (87.8 to 96.7)	95 (90.9 to 97.6)	15 (7.6 to 30.3)	0 (0 to 0.08)	H pylori positive=0†H pylori negative=4
Direct visual reading	NA	49	3	144	2	94 (84.1 to 98.8)	99 (95.1 to 99.8)	97 (94.2 to 99.2)	69 (17.2 to 494)	0.06 (0.01 to 0.17)	NA

NA=Not applicable. 

^*According to manufacturer, these cases should be considered equivocal and second stool sample should be tested. The four H pylori negative results are included in the10 false positives. 

^†According to invasive tests (histology plus urease test or culture).