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. Author manuscript; available in PMC: 2010 Jun 4.
Published in final edited form as: DNA Repair (Amst). 2009 Mar 17;8(6):739–751. doi: 10.1016/j.dnarep.2009.02.003

Fig. 4.

Fig. 4

Steady-state protein levels of Msh2 and Msh2 NLS variants in the presence and absence of Msh3 and Msh6. Proteins extracts from the strains described for Fig. 3 were analyzed using immunoblotting methods (see Materials and Methods). The nomenclature for the strains and expressed wild-type and NLS mutant proteins is the same as was described in the Fig. 3 legend panels A and B except that the msh2Δ strain with the pRS413 vector is simply designated 2Δ. After visualization of Msh2 with 12CA5 mouse α-hemaglutinin (αHA) and α-mouse IgG HRP antibodies, the membrane was re-probed with rabbit α-Kar2p polyclonal and α-rabbit IgG HRP antibodies (loading). Protein levels were quantified by densitometry using ImageJ [36]. The intensities were normalized to the loading control. The values are presented below the lanes and represent the percentage of wild-type Msh2 levels. The Msh2 Δ552 and the Msh2Δ525 Δ552 immunoblotting experiments were conducted on the same gel and membrane. Thus, the positive and negative controls are the same images reproduced for reference on the bottom panel.