Table 2.
Plasmids used in this study
| Name | Relevant Markers | Strain Number | Source |
|---|---|---|---|
| pMR5484 | RFP-kanMX4 | MR5484 | S. Clark, Rose Lab |
| pSH44 | PLEU2-(GT)16.5-URA3 TRP1/ARS CEN ampr | [29] | |
| pMR3453 | PGAL-GFP CEN4 ARS1 LEU2 ampr | MR3453 | [61] |
| pCFP-HDEL | CFP-HDEL TRP1 CEN ARS ampr | MR5214 | P. Melloy, Rose Lab |
| p3xGFP-HDEL | 3xGFP-HDEL TRP1 CEN ARS ampr | MR5029 | [62] |
| pMSH2-Δ525 | msh2-ΔNLS525 HIS3 CEN ARS ampr | MR5210 | this study |
| pMSH2-Δ552 | msh2-ΔNLS552 HIS3 CEN ARS ampr | MR5658 | this study |
| pMSH2-Δ525Δ552 | msh2-ΔNLS525 NLS552 HIS3 CEN ARS ampr | MR5586 | this study |
| pMSH2 | MSH2 HIS3 CEN ARS ampr | AG17 | [33] |
| pRS413 | HIS3 CEN ARS ampr | [63] | |
| pMSH2-C345R | msh2-C345R HIS3 CEN ARS ampr | AG208 | [33] |
| pMSH2-R542P | msh2-R542P HIS3 CEN ARS ampr | AG29 | [33] |
| pMSH2-S762Y | msh2-S762Y HIS3 CEN ARS ampr | AG462 | [33] |
| pMSH2-L521P | msh2-L521P HIS3 CEN ARS ampr | AG420 | [33] |
| pGBD-C2 | GBD TRP1 2μ ampr | [38] | |
| pGBD-MSH2 | GBD-MSH2 TRP1 2μ ampr | AG124 | [33] |
| pGBD-MSH2-C345R | GBD-msh2-C345R TRP1 2μ ampr | AG235 | [33] |
| pGBD-MSH2-R542P | GBD-msh2-R542P TRP1 2μ ampr | AG132 | [33] |
| pGBD-MSH2-S762Y | GBD-msh2-S762Y TRP1 2μ ampr | AG455 | [33] |
| pGBD-MSH2-L521P | GBD-msh2-L521P TRP1 2μ ampr | AG447 | [33] |
| pGAD-MSH6 | GAD-MSH6 LEU2 2μ ampr | AG333 | [33] |
| pBS-3xGFP–TRP1 | 3xGFP TRP1 ampr | [64] | |
| pPMSH2-GFP | PMSH2-GFP HIS3 CEN ARS ampr | This study | |
| p3xGFP | PMSH2-3xGFP HIS3 CEN ARS ampr | MR5173 | This study |
| p3xGFP-NLSSV40 | PMSH2-3xGFP-NLS SV40 HIS3 CEN ARS ampr | MR5201 | This study |
| p3xGFP-NLS525 | PMSH2-3xGFP-NLS 525 HIS3 CEN ARS ampr | MR5202 | This study |
| p3xGFP-NLS552 | PMSH2-3xGFP-NLS 552 HIS3 CEN ARS ampr | MR5203 | This study |