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. Author manuscript; available in PMC: 2010 Jun 4.
Published in final edited form as: DNA Repair (Amst). 2009 Mar 17;8(6):739–751. doi: 10.1016/j.dnarep.2009.02.003

Table 3.

Primers used in this study

Name Purpose Sequence (5′ to 3′)
MSH6-5 MSH6 disruption GGCTTCTGGTTAAGTTTGGC
MSH6-3 MSH6 disruption GCTGAATCATAGGTCAAG
MSH3-5 MSH3 disruption GTGCAATGACGATTTGAGCGGCC
MSH3-3 MSH3 disruption GCAACTTGTACAAGGCCAAGGC
PR648 Verification of 3′ kanMX junction TATGGAACTGCCTCGGTGAG
PR649 Verification of 5′ kanMX junction TCGATAGATTGTCGCACCTG
MSH6F2+ MSH6::RFP fusion CGTTTTGAAAAGTCTATTTAGTATAATTTGATGATTTACAAATCCGGTGGTGGTGGTGGTGGTCGGATCCCCGGGTTAATTAA
MSH6R1− MSH6::RFP fusion AAATAAGTAAAAATCTTACATACATCGTAAATGAAAATACGGATTCGAGCTCGTTTAAAC
MSH3F2+ MSH3::RFP fusion GCAACGGATAAACTCGCGAAATTACTATCATTGGATATCCACGGTGGTGGTGGTGGTCGGATCCCCGGGTTAATTAA
MSH3R1− MSH3::RFP fusion GTCTGATAATGCTGCATTTAGAACATACGTACCATCCGCAGGATTCGAGCTCGTTTAAAC
MSH2GFP 5′ Amplify GFP CCTAACATCAAAATCCTCAGATTAAAAGTATGAGTAAAGGAGAAGAACTT
vecGFP 3′ Amplify GFP GTGGCGGCCGCTCTAGAACTAGTGGATCCTTATTTGTATAGTTCATCCATGCC
vecMSH2 5′ Amplify PMSH2 ATCGAATTCCTGCAGCCCGGGGGATCCACCTCAACAGCTACACATTC
MSH2GFP 3′ Amplify PMSH2 AAGTTCTTCTCCTTTACTCATACTTTTAATCTGAGGATTTTGATGTTAGG
SV40NLS+ SV40 NLS 3xGFP fusion GGCATGGATGAACTATACAAACCAAAGAAGAAGAGAAAGTAAACTAGTTCTAGAGCGGCCGCCAC
SV40NLS− SV40 NLS 3xGFP fusion GTGGCGGCCGCTCTAGAACTAGTTTACTTTCTCTTCTTCTTTGGTTTGTATAGTTCATCCATGCC
2NLS525+ NLS 525 3xGFP fusion GGCATGGATGAACTATACAAACCGGACAAAAAACTGAAGTTGTAAACTAGTTCTAGAGCGGCCGCCAC
2NLS525− NLS 525 3xGFP fusion GTGGCGGCCGCTCTAGAACTAGTTTACAACTTCAGTTTTTTGTCCGGTTTGTATAGTTCATCCATGCC
2NLS552+ NLS 552 3xGFP fusion GGCATGGATGAACTATACAAACGTAAACATAAGAAGTAAACTAGTTCTAGAGCGGCCGCCAC
2NLS552− NLS 552 3xGFP fusion GTGGCGGCCGCTCTAGAACTAGTTTACTTCTTATGTTTACGTTTGTATAGTTCATCCATGCC
PR724 Sequencing NLS GFP fusions GGAAACAGCTATGACCATG
ΔNLS 525 Mutagenesis of MSH2 NLS525 GCTGAAGATCTAGGATTCGATCCGGCTGCTGCTCTGGCTTTGGAGAACCATCATCTGCATGG
ΔNLS 552 Mutagenesis of MSH2 NLS552 GACACGTAATGACGCCAAGGAGTTACGTGCTGCTGCTGCTTACATTGAGTTGTCGACAGTAAAAGC
MSH2-7 Sequencing of NLSs AAGTTGAGTTTAATGAGG