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. Author manuscript; available in PMC: 2009 Aug 17.
Published in final edited form as: In Vitro Cell Dev Biol Anim. 2008 Apr 10;44(5-6):154–161. doi: 10.1007/s11626-008-9090-4

Figure 3.

Figure 3

Removal of Wolbachia with tetracycline and effects on doubling time. (a) Toxicity curve for tetracycline. Growth of A. albopictus C7-10 cells after 4 d in E-5 medium containing various concentrations of tetracycline is expressed as a percentage of control values in the absence of tetracycline (1.9×106 cells per milliliter). Values are averaged from two counts from each of two duplicate plates; variation among counts was less than 5%. (b) DNA was extracted from Aa23E cells (passages 5, 6, 7, 8, established over a 7-wk interval) and assayed by PCR using primer pairs 99F/994R (Table 1). Tetracycline-cured cells (8T) had been maintained for 22 d in tetracycline. Base pair (bp) markers indicate band length. (c) Similar growth rates in Aa23E and Aa23E(T) cells. Solid symbols show the high and low values from a range of three to five replicate counts of nuclei from NP-40-lysed Aa23E cells; open symbols show similar data for Aa23E(T) cells. Counts ranged from 1.8×105 to 1×106 nuclei per milliliter.