Figure 5.

Comparison of cell growth in the presence and absence of tetracycline. Cells were seeded at approximately 2×10⁵ per 35-mm plate in E-20 medium, allowed to grow at 28°C, 5% CO₂, and assayed with MTT for 1 h at the indicated time points. Solid circles indicate Wolbachia-infected cells grown in the absence of tetracycline; the R² value for the computer-generated trendline was 0.950. Open circles (note superimposition of some points) indicate cells grown in the presence of tetracycline from the time of plating. The R² value for these data was 0.961.